EGS is a homobifunctional amine-specific crosslinking reagent based on NHS-ester reactive groups for selective conjugation of primary amines.
EGS contains two NHS ester groups at both ends of a 12-atom spacer that readily react towards primary amines at pH 7-9.
EGS is not water soluble and must be dissolved in an organic solvent such as DMSO or DMF prior to use.
EGS is membrane permeable and can be used for intracelular crosslinking.
The crosslink formed when using EGS can be cleaved by treatment with hydroxylamine at 35 °C at pH 8.5.


Non-cleavable and membrane permeable
Readily reacts with primary amines in the pH range 7-9

Di(N-succinimidyl) ethylene glycol disuccinate, ethylene glycol bis-(N-hydroxysuccinimidylsuccinate)


456.36 g/mol

128-132 °C

Desiccated at -20 °C under Argon. Product is shipped at ambient temperature

≥ 98%


Acetic acid/water (1:1) up to 50 mg/ml and in DMF

Order guide

Order-No.Unit SizePrice
H004N,0100100 mg50.00
H004N,0500500 mg85.00
H004N,10001 g150.00

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