GADPH

Introduction

Glyceraldehyde 3 phosphate dehydrogenase (GAPDH) is well known as one of the key enzymes involved in glycolysis. GAPDH is constitutively expressed in almost all tissues at high levels, therefore antibodies against GAPDH are useful as loading controls for Western Blotting. Some physiological factors, such as hypoxia and diabetes, increase GAPDH expression in certain cell types.

Properties/Highlights

Tool for normalizing gel loading differences and for Western blot quantification
Se
nsitive and reproducible 

Rabbit

WB, IHC

Hu Rt Ms Mk Chk Hm Sh Xenopus

1 mg/mL

37 

Western Blot dilution  1:5000 - 1:20000

Room Temperature

Store at -20°C for long-term storage (recommended)

Store at +4°C for short-term (1-2 weeks)

Avoid freeze/thaw cycle

 

Western Blot analysis of 293T(1), Rat brain(2), NIH 3T3(3), Sheep Muscle(4), Rabbit testis(5), using GADPH polyclonal antibody ABP136 diluted at 1:20000

Order guide

Order-No.Unit SizePrice
ABP136,0100100 µL260.00

Support Files

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