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Fixable viability dyes allow accurate discrimination between live and dead cells in flow cytometric analysis, based on cell membrane integrity.
The exclusion of dead cells from data allows a better identification of cell populations and provides an accurate determination of cell viability within samples.
VIV-ON fixable viability dyes allow staining of live cells and preserve the staining pattern after fixation and or permeabilization.
VIV-ON fixable viability dyes are amine reactive and cell impermeable. Dead cells with compromised cell membranes are typically labelled to a higher extend due to reaction with intracellular amines resulting in highly fluorescent dead cells. Impermeable live cells are labelled only on the cell surface and show dim fluorescence.
VIV-ON fixable viability dyes are available for the 405 (violet), 488 (blue) and 633 (red) laser lines.
Table 1: VIV-ON Fixable Viability Dye spectral properties